Invitrogen™ Phospho-Crk (Tyr251) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143673

Catalog No. PIPA5143673

Description

The antibody detects a 38 and 40 kDa* proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after akaline phosphatase treatment.

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Specifications

• Antigen: Phospho-Crk (Tyr251)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Crk
• Gene Alias: Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; c-Crk; CRK; CRK proto-oncogene, adaptor protein; CRK1; Crk3; Crk-I; CRKII; Crk-II; CrkIII; Crk-III; Crko; CSAID-binding protein; Csaids binding protein; CSBP; Csbp1; CSBP2; CSPB1; CT-10 related kinase 3; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Exip; Hog; I79_016605; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen activated protein kinase 14; mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; MXI2; p38; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38a; p38ALPHA; p38-alpha; p38alpha Exip; p38Hog; p38MAPK; PRKM14; PRKM15; Proto-oncogene c-Crk; reactive kinase; RK; SAPK2A; stress-activated protein kinase 2A; tRNA synthetase cofactor p38; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian); v-crk sarcoma virus CT10 oncogene-like protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12928, 1398, 1432, 26416, 54245, 81649
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.75 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P46108, P47811, P70618, Q16539, Q63768, Q64010
• Gene Symbols: Crk, Mapk14
• Immunogen: Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG