Invitrogen™ Phospho-CtIP (Ser327) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105748

Catalog No. PIPA5105748

Description

Antibody detects endogenous levels of CtIP only when phosphorylated at Ser327.

CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disrupted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.

Specifications

• Antigen: Phospho-CtIP (Ser327)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rbbp8
• Gene Alias: 9930104E21Rik; COM1; CtBP-interacting protein; CtIP; DNA endonuclease RBBP8; JWDS; RB binding protein 8, endonuclease; RBBP8; RBBP-8; retinoblastoma binding protein 8; retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RGD1308872; RIM; SAE2; SCKL2; Sporulation in the absence of SPO11 protein 2 homolog
• Host Species: Rabbit
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 225182, 291787, 5932
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: B1WC58, Q80YR6, Q99708
• Gene Symbols: Rbbp8
• Immunogen: A synthesized peptide derived from human RBBP8(Accession Q99708), corresponding to amino acid residues around phosphorylated Ser327.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG