Invitrogen™ Phospho-CXCR4 (Ser324, Ser325) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143677	100 μL

Catalog No. PIPA5143677 Supplier Invitrogen™ Supplier No. PA5143677

Description

Rabbit Polyclonal Antibody

The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells stimulated with calyculin A. These bands co-migrate with similar bands detected with anti-CXCR4 (a.a. 322-329) and anti-CXCR4 (a.a. 328-338) antibodies.

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

Specifications

• Antigen: Phospho-CXCR4 (Ser324, Ser325)
• Applications: Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.4 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: Cxcr4
• Gene Accession No.: O08565, P61073, P70658
• Gene Alias: b2b220Clo; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor (LCR1); chemokine receptor 4; chemokine receptor CXCR4; Cmkar4; CXC; CXC chemokine receptor; C-X-C chemokine receptor type 4; CXC motif chemokine; C-X-C motif chemokine receptor 4; CXCR4; CXC-R4; CXCR-4; D2S201E; FB22; Fusin; HM89; HSY3RR; HUMSTR; LAP3; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; pre-B-cell-derived chemokine receptor; SDF-1 receptor; Sdf1r; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; WHIM; WHIMS
• Gene Symbols: Cxcr4
• Host Species: Rabbit
• Immunogen: Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12767, 60628, 7852
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG