Invitrogen™ Phospho-Cyclin E (Thr77) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5104585

Catalog No. PIPA5104585

Description

Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr77.

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Specifications

• Antigen: Phospho-Cyclin E (Thr77)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CCNE1
• Gene Alias: AW538188; Ccne; Ccne1; CycE1; CYCLE; cyclin E; cyclin E1; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1; pCCNE1
• Host Species: Rabbit
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12447, 25729, 898
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P24864, P39949, Q61457
• Gene Symbols: CCNE1
• Immunogen: A synthesized peptide derived from human CCNE1(Accession P24864), corresponding to amino acid residues around phosphorylated Thr77.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov