Phospho-DARPP-32 (Thr75) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA538065

Catalog No. PIPA538065

Description

Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson′s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3′,5′-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

Specifications

• Antigen: Phospho-DARPP-32 (Thr75)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PPP1R1B
• Gene Alias: Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19049, 360616, 84152
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: Dulbecco′s PBS with 150mM NaCl, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q9UD71, Q60829, Q6J4I0
• Gene Symbols: PPP1R1B
• Immunogen: A synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Thr75 (A-Y-TP-P-P)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Non-human Primate, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov