Invitrogen™ Phospho-DENND3 (Ser554) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595668

Catalog No. PIPA595668

Description

Antigen Affinity Purified from Pooled Serum.

Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (PubMed:20937701). Regulates autophagy in response to starvation through RAB12 activation. Starvation leads to ULK1/2-dependent phosphorylation of Ser-472 and Ser-490, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards RAB12. Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4 (PubMed:20937701). Starvation also induces phosphorylation at Tyr-858, which leads to up-regulated GEF activity and initiates autophagy. [UniProt].

Specifications

• Antigen: Phospho-DENND3 (Ser554)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DENND3
• Gene Alias: AI447457; DENN domain containing 3; DENN domain-containing protein 3; DENN/MADD domain containing 3; Dennd3; E030003N15Rik; KIAA0870
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22898
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: ~0.25 mg/mL
• Formulation: 10mM HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: A2RUS2
• Gene Symbols: DENND3
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG