Invitrogen™ Phospho-DNA ligase IV (Thr650) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105827

Catalog No. PIPA5105827

Description

Antibody detects endogenous levels of DNA Ligase IV only when phosphorylated at Thr650.

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Specifications

• Antigen: Phospho-DNA ligase IV (Thr650)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIG4
• Gene Alias: 5830471N16Rik; DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; Lig4; LIG4S; ligase IV, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; tiny
• Host Species: Rabbit
• Purification Method: Sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 319583, 3981
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P49917, Q8BTF7
• Gene Symbols: LIG4
• Immunogen: A synthesized peptide derived from human LIG4(Accession P49917), corresponding to amino acid residues around phosphorylated Thr650.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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