Phospho-ECT2 (Thr790)Rabbit anti-Canine, Chicken, Chimpanzee, Human, Mouse, Rat, Zebrafish, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401935

Catalog No. 50-199-1877

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue. Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Specifications

• Antigen: Phospho-ECT2 (Thr790)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ECT2
• Gene Alias: AI528536; ARHGEF31; BOS_1061; Ect2; ect2 oncogene; ect2 protein; epithelial cell transforming 2; epithelial cell transforming sequence 2 oncogene; epithelial cell transforming sequence 2 oncogene protein; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; LOW QUALITY PROTEIN: protein ECT2; MGC138291; mKIAA4037; Protein ECT2
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 13605, 1894, 361921, 424983, 445406, 460853, 488172
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q07139, Q9H8V3
• Gene Symbols: ECT2
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine, Chicken, Chimpanzee, Human, Mouse, Rat, Zebrafish
• Product Type: Antibody