Invitrogen™ Phospho-EEF2K (Ser359) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143688

Catalog No. PIPA5143688

Description

The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment.

EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.

Specifications

• Antigen: Phospho-EEF2K (Ser359)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EEF2K
• Gene Alias: C86191; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; Eef2k; eEF-2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; kinase eEF2K; SMEF2K
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13631, 25435, 29904
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: O00418, O08796, P70531
• Gene Symbols: EEF2K
• Immunogen: Phospho-eEF2K (Ser-359) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-359 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG