Invitrogen™ Phospho-eIF4E (Ser209) Recombinant Rabbit Monoclonal Antibody (RM452)

Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA562157

Catalog No. MA562157

Description

This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated. This antibody may also react to mouse or rat Phospho-eIF4E (Ser209) as predicted by immunogen homology.

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Specifications

• Antigen: Phospho-eIF4E (Ser209)
• Classification: Recombinant Monoclonal
• Concentration: Conc. Not Determined
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene Accession No.: P06730
• Gene Symbols: Eif4e
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1977
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot
• Clone: RM452
• Conjugate: Unconjugated
• Gene: Eif4e
• Gene Alias: AUTS19; CBP; EG668879; EIF4E; EIF-4E; EIF4E1; EIF4EL1; Eif4e-ps; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E-like protein; eukaryotic translation initiation factor 4-like protein; If4e; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; translation initiation factor eIF-4E
• Immunogen: A phospho-peptide corresponding to human Phospho-eIF4E (Ser209).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG