Invitrogen™ Phospho-eIF4E (Thr210) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105472

Catalog No. PIPA5105472

Description

Antibody detects endogenous levels of eIF4E only when phosphorylated at Thr210.

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Specifications

• Antigen: Phospho-eIF4E (Thr210)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Eif4e
• Gene Alias: AUTS19; CBP; EG668879; EIF4E; EIF-4E; EIF4E1; EIF4EL1; Eif4e-ps; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E-like protein; eukaryotic translation initiation factor 4-like protein; If4e; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; translation initiation factor eIF-4E
• Host Species: Rabbit
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117045, 13684, 1977
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P06730, P63073, P63074
• Gene Symbols: Eif4e
• Immunogen: A synthesized peptide derived from human EIF4E(Accession P06730), corresponding to amino acid residues around phosphorylated Thr210.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov