Invitrogen™ Phospho-ELK1 (Ser389) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA537559

Catalog No. PIPA537559

Description

A suggested positive control for Western blot is HT29 cells; suggested positive control for IHC is human breast carcinoma.

ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.

Specifications

• Antigen: Phospho-ELK1 (Ser389)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ELK1
• Gene Alias: ELK 1; ELK1; Elk-1; ELK1, ETS transcription factor; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS transcription factor ELK1; ETS-like gene 1; Oncogene Elk1; tyrosine kinase (ELK1) oncogene
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13712, 2002, 314436
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: A4GTP4, P19419, P41969
• Gene Symbols: ELK1
• Immunogen: Peptide sequence around phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from Human Elk-1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG