Invitrogen™ Phospho-ERK1 (Thr202, Tyr204) Monoclonal Antibody (M206)

Catalog No.	Quantity
PIMA547641	100 μL

Catalog No. PIMA547641 Supplier Invitrogen™ Supplier No. MA547641

Description

Mouse Monoclonal Antibody

The antibody detects 42 and 44 kDa* proteins corresponding to ERK1 and ERK2 on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with EGF or calyculin A. It does not detect bands in control cells.

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

Specifications

• Antigen: Phospho-ERK1 (Thr202, Tyr204)
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M206
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: MAPK3
• Gene Accession No.: P21708, P27361, Q63844
• Gene Alias: ERK; Erk1; ERK-1; Ert2; Esrk1; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAP kinase2; MAPK 1; MAPK 3; MAPK1; MAPK2; MAPK3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; MNK1; Mtap2k; p44; p44 MAP kinase; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; pp42/MAP kinase; PRKM3
• Gene Symbols: MAPK3
• Host Species: Mouse
• Immunogen: Clone M206 was generated from a dual phosphorylated ERK1 (Thr-202/Tyr-204) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-202 and Tyr-204 in human ERK1. This sequence is conserved in rat and mouse ERK1, and is highly conserved in ERK2.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 26417, 50689, 5595
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1