Invitrogen™ Phospho-GAB1 (Tyr627) Monoclonal Antibody (T.921.2)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA514812

Catalog No. PIMA514812

Description

MA5-14812 detects Phospho-GAB1 pTyr627 in human samples. Predicted to react with mouse and rat based on sequence homology. MA5-14812 has been successfully used in Western blot applications. The MA5-14812 immunogen is synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1. MA5-14812 detects Phospho-Gab1 (Tyr627) which has a predicted molecular weight of approximately 110 kDa.

Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

Specifications

• Antigen: Phospho-GAB1 (Tyr627)
• Classification: Monoclonal
• Concentration: 20 μg/mL
• Formulation: 0.01M HEPES with 0.1mg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: Q13480
• Gene Symbols: GAB1
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: T.921.2
• Conjugate: Unconjugated
• Gene: GAB1
• Gene Alias: AA408973; AW107238; EGK_16125; GAB1; GRB2 associated binding protein 1; GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated binding protein 1 long isoform; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; OTTHUMP00000220263
• Host Species: Rabbit
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 2549
• Content And Storage: -20°C
• Form: Liquid