Invitrogen™ Phospho-GAB1 (Tyr659) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536806

Catalog No. PIPA536806

Description

This antibody detects endogenous protein at a molecular weight of 76 and 110 kDa. Purity is >95% by SDS-PAGE.

Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

Specifications

• Antigen: Phospho-GAB1 (Tyr659)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GAB1
• Gene Alias: AA408973; AW107238; EGK_16125; GAB1; GRB2 associated binding protein 1; GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated binding protein 1 long isoform; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; OTTHUMP00000220263
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14388, 2549, 361388
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide; pH 7.2
• Gene Accession No.: Q13480, Q9QYY0
• Gene Symbols: GAB1
• Immunogen: Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 659.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG