Invitrogen™ Phospho-gamma-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody (BLR053F)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA544299

Catalog No. PIMA544299

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.

Specifications

• Antigen: Phospho-gamma-H2AX (Ser139)
• Classification: Recombinant Monoclonal
• Concentration: 100 μg/mL
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
• Gene Accession No.: P16104
• Gene Symbols: H2AX
• Immunogen: Peptide surrounding phosphorylated serine 140 of human histone H2AX.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: BLR053F
• Conjugate: Unconjugated
• Gene: H2AX
• Gene Alias: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax
• Host Species: Rabbit
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 3014
• Content And Storage: 4°C
• Form: Liquid