Invitrogen™ Phospho-GRK2 (Tyr86) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5106089

Catalog No. PIPA5106089

Description

Antibody detects endogenous levels of GRK2 only when phosphorylated at Tyr86.

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

Specifications

• Antigen: Phospho-GRK2 (Tyr86)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GRK2
• Gene Alias: ADRBK1; Adrbk-1; adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; BARK; BARK1; Bark-1; beta ARK; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; betaARK1; BETA-ARK1; beta-ARK-1; EC 2.7.1.126; G protein-coupled receptor kinase 2; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2; Grk2; GRK-2
• Host Species: Rabbit
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 110355, 156, 25238
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P25098, P26817, Q99MK8
• Gene Symbols: GRK2
• Immunogen: A synthesized peptide derived from human GRK2(Accession P25098), corresponding to amino acid residues around phosphorylated Tyr86.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG