Invitrogen™ Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody

Catalog No.	Quantity
PIPA585792	100 μL

Catalog No. PIPA585792 Supplier Invitrogen™ Supplier No. PA585792

Description

Rabbit Polyclonal Antibody

Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%). Positive Control: 293T, A431, HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

Specifications

• Antigen: Phospho-GSK3 alpha/beta (Tyr279, Tyr216)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 1.91 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: GSK3A
• Gene Accession No.: P49840, P49841
• Gene Alias: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; FA; factor A; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK3; GSK-3; GSK-3 alpha; GSK-3 beta; Gsk3a; gsk-3-alpha; Gsk3b; GSK-3beta; GSK3beta isoform; serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
• Gene Symbols: GSK3A, GSK3B
• Host Species: Rabbit
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GSK3 alpha + GSK3 beta. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2931, 2932
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG