Invitrogen™ Phospho-HDAC4/HDAC5/HDAC9 (Ser246, Ser259, Ser220) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA537835

Catalog No. PIPA537835

Description

A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma.

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Specifications

• Antigen: Phospho-HDAC4/HDAC5/HDAC9 (Ser246, Ser259, Ser220)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HDAC4
• Gene Alias: AHO3; Antigen NY-CO-9; BDMR; DKFZp779K1053; HA6116; HD4; HD5; HD7; HD7b; HD9; HDAC; HDAC4; HDAC-4; HDAC5; HDAC7; HDAC7B; HDAC9; hdac-9; HDAC9B; HDAC9FL; HDACA; HDAC-A; HDRP; histone deacetylase 4; histone deacetylase 4/5-related protein; histone deacetylase 5; Histone deacetylase 7B; histone deacetylase 9; histone deacetylase A; Histone deacetylase-related protein; KIAA0288; KIAA0600; KIAA0744; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR; MITR; NY-CO-9
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10014, 9734, 9759
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P56524, Q9UKV0, Q9UQL6
• Gene Symbols: HDAC4, HDAC5, HDAC9
• Immunogen: Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov