Invitrogen™ Phospho-Histone H2B (Ser14) Recombinant Rabbit Monoclonal Antibody (RM238)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA524699

Catalog No. PIMA524699

Description

This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

Specifications

• Antigen: Phospho-Histone H2B (Ser14)
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.2-7.4
• Gene Accession No.: A0A2R8Y619, O60814, P06899, P0C1H6, P23527, P33778, P57053, P58876, P62807, Q16778, Q5QNW6, Q7Z2G1, Q8N257, Q93079, Q96A08, Q99877, Q99879, Q99880
• Gene Symbols: H2B, H2bc1, H2BC10, H2BC11, H2BC12, H2BC13, H2BC14, H2BC15, H2BC17, H2bc18, H2BC21, H2BC3, H2BC4, H2BC5, H2BC8, H2BC9, H2BE1, H2BU1, H2BW1, H2BW2, LOC102724334
• Immunogen: Phospho-peptide corresponding to Phospho-Histone H2B (Ser14).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot, Luminex, Immunocytochemistry
• Clone: RM238
• Conjugate: Unconjugated
• Gene: H2BW2
• Gene Alias: 2610022J01Rik; Acetyl-Histone H2; AV127319; bA317E16.3; CG17949; CG17949-PA; CG33868; CG33868-PA; CG33870; CG33870-PA; CG33872; CG33872-PA; CG33874; CG33874-PA; CG33876; CG33876-PA; CG33878; CG33878-PA; CG33880; CG33880-PA; CG33882; CG33882-PA; CG33884; CG33884-PA; CG33886; CG33886-PA; CG33888; CG33888-PA; CG33890; CG33890-PA; CG33892; CG33892-PA; CG33894; CG33894-PA; CG33896; CG33896-PA; CG33898; CG33898-PA; CG33900; CG33900-PA; CG33902; CG33902-PA; CG33904; CG33904-PA; CG33906; CG33906-PA; CG33908; CG33908-PA; CG33910; CG33910-PA; dJ160A22.3; dJ193B12.2; dJ221C16.3; dJ221C16.6; dJ221C16.8; dJ97D16.4; Dmel\CG17949; Dmel\CG33868; Dmel\CG33870; Dmel\CG33872; Dmel\CG33874; Dmel\CG33876; Dmel\CG33878; Dmel\CG33880; Dmel\CG33882; Dmel\CG33884; Dmel\CG33886; Dmel\CG33888; Dmel\CG33890; Dmel\CG33892; Dmel\CG33894; Dmel\CG33896; Dmel\CG33898; Dmel\CG33900; Dmel\CG33902; Dmel\CG33904; Dmel\CG33906; Dmel\CG33908; Dmel\CG33910; Dmel_CG17949; Dmel_CG33868; Dmel_CG33870; Dmel_CG33872; Dmel_CG33874; Dmel_CG33876; Dmel_CG33878; Dmel_CG33880; Dmel_CG33882; Dmel_CG33884; Dmel_CG33886; Dmel_CG33888; Dmel_CG33890; Dmel_CG33892; Dmel_CG33894; Dmel_CG33896; Dmel_CG33898; Dmel_CG33900; Dmel_CG33902; Dmel_CG33904; Dmel_CG33906; Dmel_CG33908; Dmel_CG33910; fj05g08; GL105; H2B; H2B 291A; H2B 291B; H2b 613; H2B clustered histone 1; H2B clustered histone 15; H2B clustered histone 17; H2B clustered histone 19; H2B clustered histone 2, pseudogene; H2B clustered histone 21; H2B clustered histone 5; H2B clustered histone 8; H2B GL105 antibody; H2B histone family member M; H2B histone family member W testis-specific; H2B histone family member W, testis specific; H2B histone family, member A; H2B histone family, member B; H2B histone family, member C; H2B histone family, member D; H2B histone family, member E; H2B histone family, member F; H2B histone family, member J; H2B histone family, member K; H2B histone family, member L; H2B histone family, member M; H2B histone family, member N; H2B histone family, member Q; H2B histone family, member R; H2B histone family, member S; H2B histone family, member T; H2B histone family, member U, (testis-specific); H2B histone family, member W, testis-specific; H2B I; H2B II; H2B III; H2B IV; H2B K; H2B type 12; H2B VI; H2B VII; H2B.1; H2B.1 antibody; H2B.1A; H2B.1B; H2B.2; H2B.U histone 1; H2B.W histone 1; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/g; H2B/h; H2B/I; H2B/j; H2B/k; H2B/l; H2B/n; H2B/o; H2B/q; H2B/r; H2B/S; H2B/t; H2B1; H2B1.1; H2B1.2; h2B-143; H2B2; H2b-221; H2b-291b; H2b-613; H2Bb; H2BC1; H2BC10; H2BC15; H2BC17; H2BC19; H2BC21; H2BC2P; H2BC4; H2BC5; H2BC6; H2BC7; H2BC8; H2b-f; H2BFA; H2BFAiii; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFG; H2BFH; H2BFJ; H2BFK; H2BFL; H2BFM; H2BFN; H2BFO; H2BFQ; h2bfr; H2BFS; H2BFT; H2BFU; H2BFWT; H2BGL105; H2B-I; H2B-II; H2B-III; H2B-IV; H2BJ; H2b-j; H2BK; H2BK12ac; H2BK15ac; H2BK5ac; H2b-l; H2b-n; H2BQ; H2BS14; H2BS14p; H2BU1; H2B-VI; H2B-VII; H2BW1; HIRA-interacting protein 1; HIRA-interacting protein 2; HIRIP1; HIRIP2; His2B; His2B:CG17949; His2B:CG17949-PA; His2B:CG33868; His2B:CG33868-PA; His2B:CG33870; His2B:CG33870-PA; His2B:CG33872; His2B:CG33872-PA; His2B:CG33874; His2B:CG33874-PA; His2B:CG33876; His2B:CG33876-PA; His2B:CG33878; His2B:CG33878-PA; His2B:CG33880; His2B:CG33880-PA; His2B:CG33882; His2B:CG33882-PA; His2B:CG33884; His2B:CG33884-PA; His2B:CG33886; His2B:CG33886-PA; His2B:CG33888; His2B:CG33888-PA; His2B:CG33890; His2B:CG33890-PA; His2B:CG33892; His2B:CG33892-PA; His2B:CG33894; His2B:CG33894-PA; His2B:CG33896; His2B:CG33896-PA; His2B:CG33898; His2B:CG33898-PA; His2B:CG33900; His2B:CG33900-PA; His2B:CG33902; His2B:CG33902-PA; His2B:CG33904; His2B:CG33904-PA; His2B:CG33906; His2B:CG33906-PA; His2B:CG33908; His2B:CG33908-PA; His2B:CG33910; His2B:CG33910-PA; HIST1H2B7; HIST1H2B7L3; hist1h2ba; HIST1H2BB; HIST1H2BB antibody; Hist1h2bc; HIST1H2BD; HIST1H2BE; HIST1H2BF; HIST1H2BG; Hist1h2bh; HIST1H2BI; Hist1h2bj; hist1h2bj.L; hist1h2bj.S; HIST1H2BK; HIST1H2BL
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 102724334, 114483833, 128312, 158983, 255626, 286436, 3017, 3018, 440689, 8339, 8340, 8341, 8342, 8345, 8346, 8347, 8348, 8349, 85236, 8970
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid