Invitrogen™ Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5110128

Catalog No. PIPA5110128

Description

Immunogen sequence: TDYYRK.

Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.

Specifications

• Antigen: Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Insr
• Gene Alias: 4932439J01Rik; A330103N21Rik; CD220; CD221; D630014A15Rik; D930020L01; HHF5; hyft; IGF1 Receptor; IGF-1 receptor; IGF1 Receptor beta; Igf1r; IGF-1R; IGF1R beta; IGF-I receptor; IGF-I Receptor beta; IGFIR; IGFIRC; IGFR; Igfr1; Insr; insulin like growth factor 1 receptor; insulin receptor; insulin receptor precursor; insulin receptor preproprotein; Insulin receptor subunit alpha; Insulin receptor subunit beta; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; insulin-like growth factor 1 receptor beta subunit; insulin-like growth factor I receptor; insulin-like growth factor receptor 1; insulin-like growth factor receptor type 1; insulin-like growth factor-I receptor; insulin-like growth factor-I receptor precursor; IR; IR-A; IR-B; JTK13; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16001, 16337, 24954, 25718, 3480, 3643
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.49 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P06213, P08069, P15127, P15208, P24062, Q60751
• Gene Symbols: IGF1R, Insr
• Immunogen: A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RIGF1R (NP_0008661).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG