Invitrogen™ Phospho-IRE1 alpha (Ser724) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143627	100 μL

Catalog No. PIPA5143627 Supplier Invitrogen™ Supplier No. PA5143627

Description

Rabbit Polyclonal Antibody

Specific for endogenous levels of the ~130 kDa GluR1 protein phosphorylated at Ser724. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

Specifications

• Antigen: Phospho-IRE1 alpha (Ser724)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: ∼0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: 10 mM HEPES with 100 μg/mL BSA, 50% glycerol, 150 mM NaCl and no preservative; pH 7.5
• Gene: ERN1
• Gene Accession No.: Q9EQY0
• Gene Alias: 9030414B18Rik; AI225830; C85377; endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum to nucleus signaling 1; endoplasmic reticulum to nucleus signalling 1; endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; inositol-requiring protein 1; IRE1; IRE1a; IRE1a antibody; Ire1alpha; Ire1-alpha; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; RGD1559716; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1; serine/threonine-protein kinase/endoribonuclease IRE1-like
• Gene Symbols: ERN1
• Host Species: Rabbit
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser724 of mouse IRE1, conjugated to keyhole limpet hemocyanin (KLH).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 78943
• Target Species: Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG