Invitrogen™ Phospho-IRF3 (Ser386) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121307

Catalog No. PIPA5121307

Description

Positive test controls include: HeLa, NIH/3T3. The target is usually found in the following locations: Cytoplasm, Nucleus. Immunogen sequence: ASSLE.

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

Specifications

• Antigen: Phospho-IRF3 (Ser386)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IRF3
• Gene Alias: C920001K05Rik; IIAE7; Interferon regulatory factor 3; IRF3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 292892, 3661, 54131
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.45 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P70671, Q14653
• Gene Symbols: IRF3
• Immunogen: A synthetic phosphorylated peptide around S386 of human IRF3 (NP_001562.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG