Invitrogen™ Phospho-IRS1 (Ser323) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105165

Catalog No. PIPA5105165

Description

Antibody detects endogenous levels of IRS1 only when phosphorylated at Ser323, which site historically referenced as Ser318.

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Specifications

• Antigen: Phospho-IRS1 (Ser323)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IRS1
• Gene Alias: G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
• Host Species: Rabbit
• Purification Method: Sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16367, 25467, 3667
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P35568, P35569, P35570
• Gene Symbols: IRS1
• Immunogen: A synthesized peptide derived from human IRS1(Accession P35568), corresponding to amino acid residues around phosphorylated Ser323.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG