Invitrogen™ Phospho-IRS1 (Tyr1179) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA11060

Catalog No. PA11060

Description

PA1-1060 detects phosphorylated IRS-1 (Tyr1173) in mouse and human cells. PA1-1060 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Tyr1173) from 3T3 L1 cells treated with insulin. The PA1-1060 immunogen is a synthetic phosphopeptide corresponding to residues L(1173) E N G L N (pY) I D L D L V K(1186) of human IRS-1. This immunogenic sequence is 100% conserved in humans and chickens and 85% and 87% conserved in rat and mouse, respectively. The conserved phosphorylation site corresponds to residue Y1179 in human, Y1177 in chicken, Y1173 in mouse, and Y1172 in rat. This peptide (Cat. # PEP-205) is available for use in neutralization and control experiments.

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Specifications

• Antigen: Phospho-IRS1 (Tyr1179)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IRS1
• Gene Alias: G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16367, 3667
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1 mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P35568, P35569
• Gene Symbols: IRS1
• Immunogen: Synthetic phosphopeptide corresponding to residues L(1173) E N G L N (pY) I D L D L V K(1186) of human IRS-1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG