Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA515228

Catalog No. PIMA515228

Description

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. It is not recommended to aliquot this antibody.

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

Specifications

• Antigen: Phospho-JNK1/JNK2 (Thr183, Tyr185)
• Classification: Monoclonal
• Concentration: 591 μg/mL
• Formulation: 0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and <0.02% sodium azide; pH 7.5
• Gene Accession No.: P45983, P45984, P49185, P49186, Q91Y86, Q9WTU6
• Gene Symbols: MAPK8, MAPK9
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding pThr183/Tyr185 of human SAPK/JNK
• Quantity: 200 μL
• Primary or Secondary: Primary
• Target Species: Hamster, Human, Mouse, Rat, Yeast
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: E.665.10
• Conjugate: Unconjugated
• Gene: MAPK8
• Gene Alias: AI849689; AI851083; C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; I79_012561; JNK; JNK 55; JNK/SAPK alpha; JNK1; JNK1 beta1 protein kinase; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK-46; JNK-55; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; Mapk8; Mapk9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 9; OTTHUMP00000019552; p54 gamma; p54a; p54-alpha; p54aSAPK; Phospho-JNK; PRKM; PRKM8; Prkm9; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK; SAPK 1; SAPK gamma; SAPK1; SAPK1A; SAPK1c; SAPK-alpha; stress activated protein kinase alpha II; stress-activated protein kinase 1; stress-activated protein kinase 1a; Stress-activated protein kinase 1c; stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100764964, 100771734, 116554, 26419, 26420, 50658, 5599, 5601
• Content And Storage: -20°C
• Form: Liquid