MilliporeSigma™ phospho LSD1 (Ser112), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABE1462MI	100 μL	Unconjugated

Catalog No. ABE1462MI Supplier MilliporeSigma™ Supplier No. ABE1462

Description

Rabbit Polyclonal Antibody

Lysine-specific histone demethylase 1A (UniProt Q6ZQ88; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) is encoded by the Kdm1a (also known as Aof2, Kiaa0601, Lsd1) gene (Gene ID 99982) in murine species. The FAD+-dependent, lysine-specific demethylase (LSD) LSD1 is a component of the REST corepressor (CoREST) complexes that mediate gene repression by catalyzing the demethylation of mono- and di-methylated histone H3 Lys4 (H3K4me1/2). LSD1 is also involved in gene activation process associated with nuclear receptors through demethylation of H3K9me1/2. In addition, LSD1 is known to demethylate non-histone substrates, such as p53 and Dnmt1. LSD1 is reported to be phosphorylated on Ser112 by PKCalpha and thereby mediate PKCalpha signaling-dependent circadian clock regulation. Ser112 phosphorylation induces LSD1 interaction with CLOCK:BMAL1 to facilitate E-box-dependent transcriptional activation. Knockin mice bearing Lsd1(SA/SA) alleles coding for phosphorylation-defective S112A LSD1 mutant are reported to exhibit altered circadian rhythms in locomotor behavior with attenuated rhythmic expression of core clock genes and impaired circadian clock phase resetting.

Specifications

• Antigen: phospho LSD1 (Ser112)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: Kdm1a, Aof2, Kiaa0601, Lsd1
• Gene Accession No.: Q6ZQ88
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_598633
• Test Specificity: The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.