Invitrogen™ Phospho-MAPRE3 (Ser176) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143685

Catalog No. PIPA5143685

Description

This antibody was cross-absorbed to unphospho-EB3 (Ser-176), before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier). The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Specifications

• Antigen: Phospho-MAPRE3 (Ser176)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MAPRE3
• Gene Alias: AI790651; APC binding protein; EB1 protein family member 3; EB2; EB3; EBF3; EBF3-S; End-binding protein 3; Mapre3; microtubule associated protein RP/EB family member 3; microtubule-associated protein RP/EB family member 3; microtubule-associated protein, RP/EB family, member 3; RP3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100732, 22924, 298848
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q5XIT1, Q6PER3, Q9UPY8
• Gene Symbols: MAPRE3
• Immunogen: Phospho-EB3 (Ser-176) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG