Phospho-MARK2 (Thr595)Rabbit anti-Bovine, Canine, Human, Mouse, Rat, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-1847	100 μg

Catalog No. 50-199-1847 Supplier Rockland Immunochemicals Supplier No. 600401895

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.

Specifications

• Antigen: Phospho-MARK2 (Thr595)
• Applications: ELISA, Immunofluorescence, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: MARK2
• Gene Accession No.: O08679, Q05512, Q7KZI7
• Gene Alias: ELKL motif kinase; ELKL motif kinase 1; Emk; EMK1; EMK-1; MAP/microtubule affinity regulating kinase 2; MAP/microtubule affinity-regulating kinase 2; MARK2; mark2 {ECO:0000312; MGC99619; microtubule affinity regulating kinase 2; OTTHUMP00000218338; OTTHUMP00000218339; OTTHUMP00000218340; OTTHUMP00000218343; Par-1; PAR1 homolog; PAR1 homolog b; Par1b; Par-1b; RGD:708483}; Ser/Thr protein kinase PAR-1B; serine/threonine kinase; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK2; testicular tissue protein Li 117
• Gene Symbols: MARK2
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13728, 2011, 483769, 535197, 60328
• Target Species: Bovine, Canine, Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid