Invitrogen™ Phospho-MCM4 (Ser88) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105562

Catalog No. PIPA5105562

Description

Antibody detects endogenous levels of MCM4 only when phosphorylated at Ser88.

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Specifications

• Antigen: Phospho-MCM4 (Ser88)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Mcm4
• Gene Alias: 19G; AI325074; AU045576; cb1025; Cdc21; CDC21 homolog; CDC54; DNA replication licensing factor MCM4; fc12c09; fj85g09; hCdc21; hm:zeh1616; homolog of S. pombe cell devision cycle 21; mcdc21; MCM4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin; mcm4 protein; Mcmd4; mini chromosome maintenance deficient 4 homolog; minichromosome maintenance complex component 4; minichromosome maintenance deficient 4; minichromosome maintenance deficient 4 homolog; minichromosome maintenance deficient 4 homolog (S. cerevisiae); mKIAA4003; NKCD; NKGCD; P1-CDC21; Unknown (protein for MGC:140484); wu:fc12c09; wu:fj85g09; zeh1616
• Host Species: Rabbit
• Purification Method: Sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17217, 29728, 4173
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P33991, P49717
• Gene Symbols: Mcm4
• Immunogen: A synthesized peptide derived from human MCM4(Accession P33991), corresponding to amino acid residues around phosphorylated Ser88.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG