Phospho-MEK1/2 (Ser222, Ser226) Monoclonal Antibody (17C9.E1.H2.D7.H7.D5), Rockland™

Mouse Monoclonal Antibody

Supplier:  Rockland Immunochemicals 200301GZ6S

Catalog No. 50-199-0632

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.

MEK1/mitogen activated protein kinase/ERK kinase 1/microtubule-associated protein 2 kinase is a dual specificity kinase and catalyzes both a threonine and a tyrosine residue on MAP kinases ERK1 and ERK2. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

Specifications

• Antigen: Phospho-MEK1/2 (Ser222, Ser226)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P31938, Q01986, Q02750
• Gene Symbols: Map2k1
• Immunogen: Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.
• Regulatory Status: RUO
• Gene ID (Entrez): 170851, 26395, 5604
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Clone: 17C9.E1.H2.D7.H7.D5
• Conjugate: Unconjugated
• Gene: Map2k1
• Gene Alias: CFC3; dual specificity mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase or Erk Kinase, Dual specificity mitogen-activated protein kinase kinase, involved in ras mediated vulval induction, LEThal LET-537 (42.8 kD) (mek-2); MAP kinase/Erk kinase 1; MAP2K1; map2k1.L; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; Mek1; MEK2; mek-2; MEKK1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1 L homeolog; MKK (Thr292); MKK (Thr386); MKK1; MKK2; MP2K1; Prkmk1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); XELAEV_18018447mg
• Host Species: Mouse
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 λ