Invitrogen™ Phospho-MiTF (Ser414) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105414

Catalog No. PIPA5105414

Description

Antibody detects endogenous levels of MITF only when phosphorylated at Ser414.

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

Specifications

• Antigen: Phospho-MiTF (Ser414)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MITF
• Gene Alias: BCC2; Bhlhe32; black eyed white; bw; Class E basic helix-loop-helix protein 32; CMM8; Gsfbcc2; melanocyte inducing transcription factor; melanogenesis associated transcription factor; mi; microphtalmia-associated transcription factor; microphthalmia; microphthalmia associated transcription factor isoform M-; microphthalmia transcription factor; microphthalmia-associated transcription factor; microphthalmia-associated transcription factor A; microphthalmia-associated transcription factor isoform H; microphthalmia-associated transcription factor isoform H protein; microphthalmia-associated transcription factor Trnc-1; microphthalmia-associated transcription factor Trnc-2; microphthalmia-associated transcription factor variant 1; microphthalmia-associated transcription factor variant 2; microphthalmia-associated transcription factor variant 3; microphthalmia-associated transcription factor variant MITF-M(+); MITF; MITF-H; MITF-M; MITF-M-; transcription factor, isoform 7; vit; Vitiligo; Wh; WS2; WS2A
• Host Species: Rabbit
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17342, 4286
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O75030, Q08874
• Gene Symbols: MITF
• Immunogen: A synthesized peptide derived from human MITF(Accession O75030), corresponding to amino acid residues around phosphorylated Ser414.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Monkey
• Product Type: Antibody
• Isotype: IgG