Invitrogen™ Phospho-MSK1 (Thr581) Recombinant Rabbit Monoclonal Antibody (MSK1T581-A5)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA528080	Invitrogen™ MA528080	200 μL
PIMA537014	Invitrogen™ MA537014	20 μL

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

RPS6KA5 (MSK1) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. RPS6KA5 is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential.

Specifications

• Antigen: Phospho-MSK1 (Thr581)
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 0.1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O75582, Q8C050
• Gene ID (Entrez): 314384, 73086, 9252
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1.
• Product Type: Antibody

• Clone: MSK1T581-A5
• Content And Storage: -20°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG κ
• Target Species: Human, Mouse, Rat
• Gene: Rps6ka5
• Gene Alias: 3110005L17Rik; 6330404E13Rik; 90 kDa ribosomal protein S6 kinase 5; AI854034; kinase MSK1; KS6A5; mitogen and stress-activated protein kinase 1; Msk1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase A5; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; ribosomal protein S6 kinase, polypeptide 5; RLPK; RLSK; Rps6ka5; RSKL; RSK-like protein kinase; S6K-alpha-5
• Gene Symbols: Rps6ka5
• Primary or Secondary: Primary
• Purification Method: Protein A/G