Invitrogen™ Phospho-N-cadherin/E-cadherin (Tyr820, Tyr835, Tyr860) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143660

Catalog No. PIPA5143660

Description

This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. In western blots, the antibody detects a 130 kDa* band corresponding to N-cadherin (Tyr-860) in human endothelial cells treated with pervanadate and detects a 120 kDa band corresponding to E-cadherin (Tyr-835) in human A431 cells treated with pervanadate.

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Specifications

• Antigen: Phospho-N-cadherin/E-cadherin (Tyr820, Tyr835, Tyr860)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CDH2
• Gene Alias: Cadherin-2; CD325
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1000, 12558, 83501
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P15116, P19022, Q9Z1Y3
• Gene Symbols: CDH2
• Immunogen: Phospho-N-Cadherin (Tyr-860) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 860 in human N-cadherin. This sequence is conserved in human E-cadherin (Tyr-835), P-cadherin (Tyr-782), R-cadherin (Tyr-870), and has significant homology to VE-Cadherin (Tyr-733).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG