Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (4NIT4KK), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 46907841

Catalog No. 46-907-841

Description

Description: This 4NIT4KK monoclonal antibody recognizes human and mouse p38 mitogen-activated protein kinase (MAPK) when phosphorylated on T180/Y182. p38 MAPK belongs to a family of conserved serine/threonine protein kinases that are phosphorylated and activated in response to numerous stress stimuli including TLR ligands (such as LPS), osmotic shock, heat shock, UV irradiation, and inflammatory cytokines. There are four p38 MAPK members that include p38 alpha, p38 beta, p38 gamma, and p38 delta. The primary activators of p38 MAPK are MKK3/4 and MKK6. Several downstream targets of p38 MAPK have been identified including MK2/3, p53, ATF-2, and ETS1. p38 MAPK can be negatively regulated by the chemical inhibitor SB203580. Specificity of this 4NIT4KK clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This 4NIT4KK antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4NIT4KK antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Specifications

• Antigen: Phospho-p38 MAPK (Thr180, Tyr182)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P47811, Q16539
• Gene Symbols: MAPK14
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1432, 26416
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 4NIT4KK
• Conjugate: PerCP-eFluor 710
• Gene: MAPK14
• Gene Alias: Crk1; CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Exip; Hog; MAP kinase 11; MAP kinase 13; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAPK 11; MAPK 13; MAPK 14; Mapk11; Mapk13; MAPK-13; Mapk14; MAX-interacting protein 2; mitogen activated protein kinase 11; mitogen activated protein kinase 13; mitogen activated protein kinase 14; mitogen-activated protein kinase 11; Mitogen-activated protein kinase 13; mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38 delta; mitogen-activated protein kinase p38-2; MXI2; p38; p38 alpha; p38 delta MAP kinase; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38-2; p38a; p38ALPHA; p38-alpha; p38alpha Exip; P38b; p38Beta; p38beta2; p38delta; p38Hog; p38MAPK; Prkm11; PRKM13; PRKM14; Prkm15; protein kinase, mitogen activated kinase, 11, p38beta; reactive kinase; RK; SAPK/Erk/kinase 4; SAPK2; SAPK2A; SAPK2B; SAPK4; Serk4; Stress-activated protein kinase 2a; Stress-activated protein kinase 2b; Stress-activated protein kinase 4; stress-activated protein kinase-2; stress-activated protein kinase-2b; tRNA synthetase cofactor p38
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.