Invitrogen™ Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (C.7.8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515218

Catalog No. PIMA515218

Description

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. It is not recommended to aliquot this antibody.

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Specifications

• Antigen: Phospho-p38 MAPK (Thr180, Tyr182)
• Classification: Monoclonal
• Concentration: 156 μg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: P47811, P70618, Q16539
• Gene Symbols: MAPK14
• Immunogen: Synthetic phosphopeptide corresponding to residues around pThr180/Tyr182 of human p38 MAPK.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Yeast
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: C.7.8
• Conjugate: Unconjugated
• Gene: MAPK14
• Gene Alias: 186F5S; anon-sts23; BG:DS00797.3; CG7393; CG7393-PA; CRK1; CSAID-binding protein; Csaids binding protein; CSBP; Csbp1; Csbp2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Dm p38b; Dmel\CG7393; Dmel_CG7393; Dmp38b; Dp38; D-p38; D-p38 MAPK; Dp38b; D-p38b; EC 2.7.11.24; ERK1; ERT2; ESTS:186F5S; etID18993.8; Exip; fk28c03; hm:zeh1243; Hog; I79_016605; LD24658p; MAP 14; MAP kinase 1; MAP kinase 11; MAP kinase 14; MAP kinase 14A; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38a; MAP kinase p38alpha; MAP kinase p38b; MAPK; MAPK 11; MAPK 14; MAPK 14A; MAPK1 antibody; Mapk11; Mapk14; mapk14a; MAX-interacting protein 2; mitogen activated protein kinase 11; mitogen activated protein kinase 14; mitogen-activated protein kinase 11; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14a; Mitogen-activated protein kinase p38 alpha; mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; mitogen-activated protein kinase p38a; Mitogen-activated protein kinase p38b; MK14; Mpk34C; Mxi2; OTTHUMP00000216563; OTTHUMP00000216566; P38; p38 alpha; p38 alpha/Mxi2; p38 beta; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38 mitogen-activated protein kinase; p38-2; p38a; p38ALPHA; p38-alpha; p38alpha Exip; P38B; p38b MAP kinase; p38beta; p38beta2; p38b-PA; p38Hog; p38Kb; p38MAPK; PRKM11; Prkm14; Prkm15; protein kinase, mitogen activated kinase, 11, p38beta; reactive kinase; RK; RP1-179N16.5; Sapk2; Sapk2A; SAPK2b; stress-activated p38b MAP kinase; stress-activated protein kinase 2A; Stress-activated protein kinase 2b; stress-activated protein kinase-2; stress-activated protein kinase-2b; tRNA synthetase cofactor p38; wu:fk28c03; zeh1243; zp38a
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1432, 26416, 81649
• Content And Storage: -20°C
• Form: Liquid