Invitrogen™ Phospho-p53 (Ser392) Monoclonal Antibody (FP3.2 [FPS392])

Catalog No.	Quantity
01-676-170	100 μg

Catalog No. 01-676-170 Supplier Invitrogen™ Supplier No. MA119424

Description

Mouse Monoclonal Antibody

This antibody reacts with human p53 tumor suppressor intracellular protein phosphorylated at CKII site (Ser 392). For IHC staining, high temperature antigen retrieval (microwave, pressure cooker) is recommended, using 10 mM citrate buffer, pH 6.0, or 1 mM EDTA-NaOH buffer, pH 8.0.

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Specifications

• Antigen: Phospho-p53 (Ser392)
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: FP3.2 [FPS392]
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15 mM sodium azide; pH 7.4
• Gene: Tp53
• Gene Accession No.: P04637
• Gene Alias: Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3
• Gene Symbols: Tp53
• Host Species: Mouse
• Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7157
• Target Species: Human
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1