Invitrogen™ Phospho-PAK6 (Ser165) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143735

Catalog No. PIPA5143735

Description

This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa* band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-PAK6 (Ser-165) peptide and also by treatment with lambda phosphatase.

PAK6 is a serine/threonine kinase expressed in the testis and prostate. It associates with both the estrogen and androgen receptors. The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

Specifications

• Antigen: Phospho-PAK6 (Ser165)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Pak6
• Gene Alias: 4732456M09; p21 (CDKN1A)-activated kinase 6; p21 (RAC1) activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated kinase 6; PAK5; PAK-5; PAK6; PAK-6; Serine/threonine-protein kinase PAK 6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56924
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.25 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q9NQU5
• Gene Symbols: Pak6
• Immunogen: PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG