Phospho-Paxillin (Tyr118) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen 44722G

Catalog No. 44-722-G

Description

The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 118. Positive controls used with this antibody in western blotting were normal mouse mammary gland (NMuMG) cells, NMuMG transfected with EGFP-tagged paxillin +/- TGFbeta, and human epidermoid carcinoma cells (A431) +/- EGF.

Phospho-Paxillin (Tyr118) Polyclonal antibody specifically detects Phospho-Paxillin (Tyr118) in Human, Mouse samples. It is validated for Immunohistochemistry, Western Blot.

Specifications

• Antigen: Phospho-Paxillin (Tyr118)
• Classification: Polyclonal
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene Accession No.: P49023, Q8VI36
• Gene Symbols: PXN
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Western Blot
• Conjugate: Unconjugated
• Gene: PXN
• Gene Alias: adaptor protein; AW108311; AW123232; FLJ16691; hCG_1778014; I79_001229; myocardial ischemic preconditioning associated protein 7; Pax; PAX-1; PAXI; Paxillin; paxillin a; paxillin alpha; pxn; pxna; testicular tissue protein Li 134; TGFB1I1; transforming growth factor beta 1 induced transcript 1; wu:fw71f12
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19303, 5829
• Content And Storage: -20°C
• Form: Liquid