Phospho-Pdcd4 (Ser457) Monoclonal Antibody (9G6), Rockland™

Mouse Monoclonal Antibody

Supplier:  Rockland Immunochemicals 200301964S

Catalog No. 50-199-0547

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

Specifications

• Antigen: Phospho-Pdcd4 (Ser457)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q53EL6, Q61823, Q9JID1
• Gene Symbols: PDCD4
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
• Regulatory Status: RUO
• Gene ID (Entrez): 18569, 27250, 64031
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 9G6
• Conjugate: Unconjugated
• Gene: PDCD4
• Gene Alias: D19Ucla1; Death up-regulated gene protein; death-upregulated; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; PDCD4; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11-348N5.4; Tis; Topoisomerase-inhibitor suppressed protein
• Host Species: Mouse
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, X. laevis
• Product Type: Antibody
• Isotype: IgG1 κ