Invitrogen™ Phospho-PDPK1 (Ser241) Monoclonal Antibody (K.424.2)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA514780

Catalog No. PIMA514780

Description

It is not recommended to aliquot this antibody.

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Specifications

• Antigen: Phospho-PDPK1 (Ser241)
• Classification: Monoclonal
• Concentration: 62 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: O15530, O55173, Q9Z2A0
• Gene Symbols: Pdpk1
• Immunogen: Synthetic phosphopeptide corresponding to residues around pSer241 of human PDK1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunoprecipitation, Western Blot
• Clone: K.424.2
• Conjugate: Unconjugated
• Gene: Pdpk1
• Gene Alias: 3-phosphoinositide dependent protein kinase 1; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PDK1; PDPK1; PDPK2; PDPK2P; Pkb kinase; PkB kinase like gene 1; PkB-like 1; PRO0461; Protein kinase B kinase
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18607, 5170, 81745
• Content And Storage: -20°C
• Form: Liquid