Invitrogen™ Phospho-PKC alpha (Ser657, Tyr658) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143743	100 μL

Catalog No. PIPA5143743 Supplier Invitrogen™ Supplier No. PA5143743

Description

Rabbit Polyclonal Antibody

This antibody detects an 82kDa* protein corresponding to the molecular mass of phosphorylated PKCalpha on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were observed in human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCalpha and PKCbeta but not other PKC isoforms in rat brain lysate.

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Specifications

• Antigen: Phospho-PKC alpha (Ser657, Tyr658)
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: PRKCA
• Gene Accession No.: P05696, P10102, P17252
• Gene Alias: AAG6; aging-associated gene 6; AI875142; EC 2.7.11.13; FHC; FTH; FTHL6; im:7139045; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; Pkca; PKC-A; PKC-alpha; PKC-III; PKRCA; PLIF; PRKACA; Prkca; prkcaa; protein kinase C; protein kinase C alpha; protein kinase C alpha type; protein kinase C alpha type; protein kinase C, alpha, a; protein kinase C, alpha; protein kinase C, alpha, a; protein kinase c-alpha; si:dkey-8l19.1; Unknown (protein for IMAGE:7139045); unnamed protein product
• Gene Symbols: PRKCA
• Host Species: Rabbit
• Immunogen: Phospho-PKCalpha (Ser-657/Tyr-658) synthetic peptide corresponds to amino acid residues around serine 657 and tyrosine 658 of human PKCalpha. This sequence is similar to the conserved sites in rat and mouse, and has high homology to dual sites in human, rat, and mouse PKCbetaI (Ser-661/Tyr-662) and PKCgamma (Thr-674/Tyr-675).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100037720, 24680, 5578
• Target Species: Human, Rabbit, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG