Invitrogen™ Phospho-PKC Pan (Thr497) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536757

Catalog No. PIPA536757

Description

This antibody detects endogenous levels of PKC alpha, beta-I, beta-II, gamma, delta, epsilon, eta, zeta and theta isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKC alpha. Purity is >95% by SDS-PAGE.

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ( alpha, betaI, betaII and gamma) and novel (n) PKC isoforms ( delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.

Specifications

• Antigen: Phospho-PKC Pan (Thr497)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Prkca
• Gene Alias: 14 - 3 - 3 - zeta isoform; 14-3-3-zetaisoform; 2310021H13Rik; 5830406C15Rik; A130035A12Rik; A130082F03Rik; AAG6; aging-associated gene 6; AI098070; AI385711; AI427505; AI875142; ALPS3; aPKClambda; aPKC-lambda/iota; aPKCzeta; atypical protein kinase C; atypical protein kinase C-lambda/iota; AW494342; C80388; CVID9; D14Ertd420e; DXS1179E; MAY1; mKIAA4165; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC; PKC theta; PKC zeta; PKC[d]; PKC[e]; PKC-0; PKC2; Pkca; PKC-A; PKC-alpha; Pkcb; PKC-B; PKC-beta; Pkcc; PKCD; PKCdelta; Pkce; Pkcea; PKCepsilon; PKCG; PKCgamma; PKC-gamma; Pkch; PKCI; PKCL; PKC-L; PKClambda; Pkcq; PKCtheta; PKC-theta; Pkcz; PKC-ZETA; PRKACA; Prkc; PRKCA; Prkcb; Prkcb1; Prkcb2; Prkcc; Prkcd; Prkce; PRKCG; Prkch; PRKCI; PRKCL; PRKC-lambda/iota; Prkcq; PRKCT; Prkcz; protein kinase C alpha; protein kinase C alpha type; protein kinase C beta; protein kinase C beta I; protein kinase C beta II; protein kinase C beta type; protein kinase C beta-II; protein kinase C delta; protein kinase C delta type; Protein kinase C delta type catalytic subunit; Protein kinase C delta type regulatory subunit; protein kinase C delta variant IX; protein kinase C delta VIII; protein kinase C epsilon; protein kinase C epsilon type; protein kinase C eta; protein kinase C eta type; protein kinase C gamma; protein kinase C gamma type; protein kinase C iota; protein kinase C iota type; protein kinase C theta; protein kinase C theta type; protein kinase C type I (gamma type); protein kinase C zeta; protein kinase C zeta subspecies; protein kinase C zeta type; protein kinase C, alpha; protein kinase C, beta; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, epsilon; protein kinase C, eta; protein kinase C, gamma; protein kinase C, iota; protein kinase C, lambda; protein kinase C, theta; protein kinase C, zeta; protein kinase C[d]; protein kinase c-alpha; protein kinase C-eta; r14-3-3; R74924; R75156; RATPKCI; SCA14; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD; zetaPKC
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 170538, 18750, 18751, 18752, 18753, 18754, 18755, 18759, 18761, 18762, 24680, 24681, 25023, 25522, 29340, 5578, 5579, 5580, 5581, 5582, 5583, 5584, 5588, 5590, 81749, 84006, 85420
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: F1M7Y5, P05129, P05696, P05771, P09215, P09216, P09217, P16054, P17252, P20444, P23298, P24723, P28867, P41743, P63318, P63319, P68403, P68404, Q02111, Q02156, Q02956, Q04759, Q05513, Q05655, Q62074, Q64617, Q9WTQ0
• Gene Symbols: Prkca, PRKCB, PRKCD, PRKCE, PRKCG, Prkch, Prkci, PRKCQ, PRKCZ
• Immunogen: Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG