Invitrogen™ Phospho-PKC theta (Ser695) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529029

Catalog No. PIPA529029

Description

Recommended positive controls: HeLa, HepG2, Molt-4, Raji, MCF7 (500nM PMA treatment for 1hr). Predicted reactivity: Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

Specifications

• Antigen: Phospho-PKC theta (Ser695)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKCQ
• Gene Alias: A130035A12Rik; AW494342; EC 2.7.11.13; kinase nPKC-theta; KPCT; MGC126514; MGC141919; nPKC-theta; PKC theta; PKC-0; Pkcq; PKCtheta; PKC-theta; PRKCQ; PRKCT; protein kinase C theta; protein kinase C theta type; protein kinase C, theta; RP11-563J2.1; si:ch211-239o15.1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5588
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.66 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q04759
• Gene Symbols: PRKCQ
• Immunogen: Synthetic peptide corresponding to a region within amino acids 641 and 702 of Human PKC theta-phospho-S695.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov