Invitrogen™ Phospho-PLCG1 (Tyr775) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143744

Catalog No. PIPA5143744

Description

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-PLCgamma1 (Tyr-775) peptide. The antibody detects a 150 kDa* protein in human Jurkat and A431 cells treated with pervanadate, but is not observed in untreated cells.

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

Specifications

• Antigen: Phospho-PLCG1 (Tyr775)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PLCG1
• Gene Alias: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; AI894140; Cded; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; NCKAP3; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-1; phospholipase C gamma 1; phospholipase C, gamma 1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; Phospholipase C2; phospholipase C-gamma-1; Phospholipase C-II; PLC gamma1; PLC1; Plc-1; PLC148; PLC-148; PLC2I; Plcg1; Plcg-1; PLCgamma1; Plc-gamma1; PLC-gamma-1; PLC-II; PPLCA; triphosphoinositide phosphodiesterase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18803, 25738, 5335
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P10686, P19174, Q62077
• Gene Symbols: PLCG1
• Immunogen: PLCgamma1 (Tyr-775) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 775 in human PLCgamma1. This sequence has high homology to the conserved site in rat and mouse PLCgamma1, and has low homology to PLCgamma2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG