Invitrogen™ Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1413

Catalog No. MA1-413

Description

MA1-413 detects phospho-progesterone receptor (pSer190) from human samples. MA1-413 has been successfully used in Western blot, immunohistochemistry, ELISA, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer190) A and B from T47D cell and human breast carcinoma extracts. Immunohistochemical staining of phospho-progesterone receptor (pSer190) (paraffin samples) can be performed using MA1-413. The MA1-413 immunogen is a synthetic peptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Specifications

• Antigen: Phospho-Progesterone Receptor (Ser190)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P06401
• Gene Symbols: PGR
• Immunogen: Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1154
• Conjugate: Unconjugated
• Gene: PGR
• Gene Alias: 9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 5241
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid