Invitrogen™ Phospho-RRAS (Tyr66) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5118783

Catalog No. PIPA5118783

Description

Antibody detects endogenous levels of R-Ras only when phosphorylated at Tyr66.

The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signal pathways control such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that control eukaryotic cell function by cycling between two interconvertible forms, a GDP-bound inactive form and a GTP-bound active form. The binding of GTP leads to a conformational change in the downstream effector-binding domain of the G-protein. The small GTPases are monomeric G-proteins with molecular masses over the range 20-30 kDa. Ras is attached to the cell membrane by prenylation. Mutations in the Ras family of proto-oncogenes (comprising H-Ras, N-Ras and K-Ras) are very common, being found in 20% to 30% of all human tumours. The frequency of RAS mutations is among the highest for any gene in human cancer. K-ras mutations occur frequently in non-small-cell lung, colorectal, and pancreatic carcinomas; H-ras mutations are common in bladder, kidney, and thyroid carcinomas; N-ras mutations are found in melanoma, hepatocellular carcinoma, and hematologic malignancies.

Specifications

• Antigen: Phospho-RRAS (Tyr66)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RRAS
• Gene Alias: AI573426; Harvey rat sarcoma oncogene, subgroup R; Harvey rat sarcoma virus oncogene, subgroup R; oncogene RRAS; p23; ras family small GTP binding protein R-Ras; ras-related protein R-Ras; Ras-related protein R-RAS (P23); related RAS viral (r-ras) oncogene homolog; RRAS
• Host Species: Rabbit
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20130, 361568, 6237
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: D3Z8L7, P10301, P10833
• Gene Symbols: RRAS
• Immunogen: A synthesized peptide derived from human RRAS(Accession P10301), corresponding to amino acid residues around phosphorylated Tyr66.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG