Invitrogen™ Phospho-SHP-1 (Tyr536) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143763

Catalog No. PIPA5143763

Description

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-SHP1 (Tyr-536) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate, but is not observed in control cells.

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

Specifications

• Antigen: Phospho-SHP-1 (Tyr536)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PTPN6
• Gene Alias: 70Z-SHP; EC 3.1.3.48; hcp; Hcph; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; HPTP1C; me; motheaten; protein tyrosine phosphatase, non-receptor type 6; protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; Ptp1C; PTP-1C; Ptph6; PTPN6; PTPTY-42; SH2 phosphatase 1; SHP1; Shp-1; SHP-1L; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 116689, 15170, 5777
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P29350, P29351, P81718
• Gene Symbols: PTPN6
• Immunogen: Phospho-SHP1 (Tyr-536) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG