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Invitrogen™ Phospho-SHP-1 (Tyr536) Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA5143763
Description
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-SHP1 (Tyr-536) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate, but is not observed in control cells.
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
Specifications
Phospho-SHP-1 (Tyr536) | |
Polyclonal | |
Unconjugated | |
PTPN6 | |
70Z-SHP; EC 3.1.3.48; hcp; Hcph; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; HPTP1C; me; motheaten; protein tyrosine phosphatase, non-receptor type 6; protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; Ptp1C; PTP-1C; Ptph6; PTPN6; PTPTY-42; SH2 phosphatase 1; SHP1; Shp-1; SHP-1L; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6 | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
116689, 15170, 5777 | |
-20°C, Avoid Freeze/Thaw Cycles | |
Liquid |
Western Blot | |
1 mg/mL | |
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide | |
P29350, P29351, P81718 | |
PTPN6 | |
Phospho-SHP1 (Tyr-536) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1. | |
100 μL | |
Primary | |
Human, Mouse, Rat | |
Antibody | |
IgG |
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