Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-2293	100 μg
50-199-2294	25 μL

Catalog No. 50-199-2293 Supplier Rockland Immunochemicals Supplier No. 600401K09

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

Specifications

• Antigen: Phospho-SMAD2 (Ser465, Ser467)
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.94 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: SMAD2
• Gene Accession No.: Q15796
• Gene Alias: 7120426M23Rik; BOS_22266; EGK_09664; hMAD-2; hSMAD2; JV18; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Madh2; Madr2; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic-like protein 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2; Smad-2; Smad2Deltaexon3
• Gene Symbols: SMAD2
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4087
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid