Phospho-SMAD3 (Ser423, Ser425) Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-194-3861	Rockland Immunochemicals 600401919	100 μg
50-199-1863	Rockland Immunochemicals 600401919S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Specifications

• Antigen: Phospho-SMAD3 (Ser423, Ser425)
• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Bovine, Chicken, Human, Mouse, Pig, Rat, X. laevis, Zebrafish
• Gene Accession No.: P84022, P84023, P84024, P84025, Q8BUN5
• Gene ID (Entrez): 17127, 25631, 378633, 395132, 397260, 4088, 515125, 58092
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1.1 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: AU022421; BOS_10264; DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; MAD-3; Madh3; madh3a; madh3-A; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD family member 3 L homeolog; SMAD family member 3a; SMAD, mothers against DPP homolog 3; Smad3; smad3.L; Smad3a; transcription factor; transcription factor Smad3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3
• Gene Symbols: Smad3, smad3.L, smad3a
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: Smad3